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KMID : 0360419750110020041
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Korean Journal of Pharmacology
1975 Volume.11 No. 2 p.41 ~ p.46
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EVALUATION AS A BIOASSAY PREPARATION OF TORTOISE INTESTINE FOR PROSTAGLANDIN E₁
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Hong Ki-W.
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Abstract
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The isolated strips of tortoise intestine are evaluated as a test organ for bioassay of prostaglandin E₁. This preparation responded highly sensitively to PGE₁ and PGE₂ in picogram concentration range. The mean slope and the value of precision index among the doses of 0.1, 0.3 and 0.5ng/ml in final concentration were 37.7 and 0.143, respectively. And this was relatively insensitive to different prostaglandins; E₁/E₂??1, E₁/A₂~50 and E₁/F_(2α)~100, and showed the dual responses to 5-hydroxytry-ptamine and histamine; initial contraction followed by relaxation. The dose-ratio inducing the relative equal contraction height for PGE₁, acetylcholine, caerulein, angiotensin and barium chloride was 0.4 : 50 : 25 : 10 : 100 in this order. These results suggest that the intestinal strips of the tortoise are suitable for bioassay of prostaglandin E₁ and E₂ between the doses of 0.1 and 1.0 ng/ml level in the tissue extracts.
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